How important is it to separate the Pre and Post PCR Labs when performing SARS-CoV-2 testing? One respondent says that the type of separation required depends on the method the lab is using. For fully automated real-time PCR assays, no special facilities are needed. However, if a lab is using a more manual method, it’s better to have a separate room for nucleic acid extraction and to do the reaction setup in a dead-air box. And if the method requires post-PCR analysis, it’s best to use the three-room set-up described in CLSI MM19.