Sample preparation is one of the greatest sources of variability in bioanalytical and clinical research methods, particularly for protein quantification methods which often contain multiple steps; each one contributing to variability.
The issue of variability is of particular concern when test methods are transferred between sites, or even between labs within the same organization. And laboratorians are well aware that the resulting method must meet acceptable accuracy and precision guidelines as well as provide reliable and reproducible results for critical decision-making.
This webinar will focus on the implementation and benefits of generic, kitted methods to streamline and standardize common protein quantification workflows, while reducing variability and delivering accurate results. Several examples will be provided to demonstrate the impact of these methods on controlling and reducing variability.
Lab directors, lab managers, clinical laboratory scientists, and laboratory technicians involved with transferring protein quantification from immunoassay to LC/MS, or those new to protein quantification by LC/MS. Scientists and other professionals who want to better understand the fundamentals of protein quantification by LC/MS and how to implement a reliable standardized approach will also benefit from this webinar.
After attending this webinar, you should be able to:
- Understand and appreciate the importance of standardization in protein quantification
- Have a working knowledge of a thoroughly researched approach to absolute protein quantification
Describe affinity purification options for protein level purification and SPE options for signature peptide purification
- Evaluate and select the appropriate level of sample preparation, and specific type of sample prep depending on the sensitivity requirements of your assay
- List the steps of digestion workflows and explain the impact on final quantitative results
Erin Chambers, PhD
Director, Technology Advancement Group and Principal Scientist