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Certificate Program

LC-MS/MS Troubleshooting for the Clinical Laboratory Certificate Program 2018

  • Credit: 11.0 ACCENT
  • Level: Intermediate

Price: $550.00

Member Price: $275.00

Rating: Member Average


Earn 11 ACCENT® continuing education credits and a certificate of completion for successfully completing the program before the access end date.

The program is composed of nine courses, listed below. Each course can be completed online in 1-3 hours and contains a lecture, reference materials and a quiz. Communicate questions or comments using the program discussion board.

  • System Suitability Testing and Preventive Maintenance
  • Sample Preparation
  • Liquid Chromatography for Mass Spectrometry: Operations and Troubleshooting
  • Mass Spectrometry Troubleshooting
  • Gas Chromatography Mass Spectrometry (GC/MS)
  • Data Review: Going from Peaks to Numbers
  • Quality Control and Quality Assurance for LC-MS/MS; Hydrolysis and Derivatization
  • Troubleshooting Calibration for Clinical Mass Spectrometry
  • Dealing with Change

Certificates of completion are awarded online upon successful completion of the program. Graduates are recognized on a register located on the certificate program web page.

Please note that the completion of an online certificate program should be listed within the education section of a resume or CV. It is not equivalent to completing a board certification process.

Speaker Presentation

Launch Year: 2016

This nine-course program was developed for clinical laboratory professionals with experience using tandem mass spectrometry (LC-MS/MS) and liquid chromatography mass spectrometry (LC-MS) for laboratory analyses. It would benefit technologists who wish to improve their technical skillset, as well as directors or supervisors who would like a course on issues that affect mass spectrometry testing operations. The content of this program is at the intermediate level and covers the resolution of technical issues with HPLC and mass spectrometry hardware and related mass spec assay troubleshooting and assay maintenance, quality control as well as common post-analytical problems. This program is appropriate for learners who have first-hand exposure to the technology through prior use.

After completing this program, participants will be able to:

  • Review the goal and criteria of system suitability testing and preventative maintenance Learn the sample preparation steps to maintain sample integrity, approach batch building, extraction precision and documentation.
  • Review liquid chromatography fundamentals, system suitability tests, LC maintenance and troubleshooting.
  • Identify and use QA tools to develop troubleshooting and maintenance plans for mass spectrometry technology.
  • Discuss the components of gas chromatography mass spectrometry, its advantages and limitations, as well as troubleshooting techniques.
  • Learn best practices for integrating peaks and how to implement protocols for manual peak integration. Understand how to calculate and assess relevant quality assurance metrics, and how much data to review.
  • Identify sources and types of QC matrix best suited to LC-MS/MS assays, and list quality indicators for data analysis. Identify the inherent QA/QC challenges of deconjugation and derivatization reactions, and specify appropriate strategies to work around these.
  • Understand the range of analytical components that affect calibration of assays, describe the implications of nonlinear calibration curves, and identify steps in calibrator preparation and storage that affect accuracy.
  • Describe how clinical LC-MS/MS labs handle lot changes, instrument service and decommission, longitudinal and source material changes, analytical changes in testing methodology, changes to software and personnel.

These expert faculty developed and contributed to the content in this program

Y. Victoria Zhang, PhD, DABCC, Faculty Chair
University of Rochester Medical Center, Rochester, NY

Brian Rappold
Collinsville, IL

Alan Rockwood, PhD, DABCC
ARUP Laboratories, Salt Lake City, UT

Uttam Garg, PhD, DABCC (CC, TC) FACB
Childrens Mercy Hospital, Kansas City, MO

Joshua Hayden, PhD, DABCC FACB
Weill Cornell Medical College, New York, NY

Julia Drees, PhD, DABCC
Kaiser Permanente, Berkeley, CA

Sarah Shugarts, PhD, DABCC (CC, TC)
Kaiser Permanente, Berkeley, CA

Stephen Master, MD, PhD, FACB
Weill Cornell Medical College, New York, NY

Autumn Breaud, PhD
The Johns Hopkins Hospital, Baltimore, MD


Open Enrollment until December 31, 2018
Program Access from January 1, 2018 until July 15, 2019

Fee: $275 AACC Member/$550 Non-member

Course Number: 11794

11 ACCENT® continuing education credit

  • If you purchase the program on our website, order processing is immediate.
  • If you purchase the program over the telephone, order processing will take 3-4 business days.
  • If you purchase the program via US Post or fax, please allow 2 weeks for order processing.
  • In all cases, program access information will be sent in an email titled AACC Order Confirmation once the order process is complete. Haven’t received your access information? We recommend checking your Spam/Junk Mail in case the confirmation email is filtered there. If you cannot locate the email, please contact AACC Customer Service.


ACCENT® credit is offered. AACC is an approved provider of continuing education for clinical laboratory scientists in the states of California, Florida, Louisiana, Montana, Nevada, North Dakota, Rhode Island, and West Virginia.


You must have Internet access and Internet browser software installed on your computer to view the e-learning materials in this course, as well as Adobe Reader. We recommend the following Internet browsers and browser settings.

  • Internet Explorer version 11 or higher
  • Mozilla Firefox version 33 or higher
  • Google Chrome version 36 or higher
  • Apple Safari version 6 or higher


You will need speakers attached to your computer to hear the audio files associated with some of the course materials.


For best viewing, your screen resolution should be 1024 x 768 or higher.


Cancellations and refunds are not available for e-learning courses.


1/1/2018 12:00 AM Until 12/31/2018 11:59 PM