Mass Spectrometry for the Clinical Laboratory
is an accessible guide to mass spectrometry and the development, validation, and
implementation of the most common assays seen in clinical labs. It provides
readers with practical examples for assay development, and experimental design
for validation to meet CLIA requirements, appropriate interference testing,
measuring, validation of ion suppression/matrix effects, and quality control.
These tools offer guidance on what type of instrumentation is optimal for each
assay, what options are available, and the pros and cons of each. Readers will
find a full set of tools that are either directly related to the assay they want
to adopt or for an analogous assay they could use as an example.
Written by expert users of the most common assays found in a clinical
laboratory (clinical chemists, toxicologists, and clinical pathologists
practicing mass spectrometry), the book lays out how experts in the field have
chosen their mass spectrometers, purchased, installed, validated, and brought
them on line for routine testing.
The early chapters of the book covers what the practitioners have learned
from years of experience, the challenges they have faced, and their
recommendations on how to build and validate assays to avoid problems. These
chapters also include recommendations for maintaining continuity of quality in
testing. The later parts of the book focuses on specific types of assays
(therapeutic drugs, Vitamin D, hormones, etc.). Each chapter in this section has
been written by an expert practitioner of an assay that is currently running in
his or her clinical lab.