AACC uses Cookies to ensure the best website experience. Continuing without changing Cookie settings assumes you consent to our use of cookies on this device. You can change these settings at any time, but that may impair functionality on our websites. Review our cookie and privacy policy

Certificate Program

LC-MS/MS Troubleshooting for the Clinical Laboratory Certificate Program

  • Credit: 11.0 ACCENT
  • Level: Intermediate

Price: $550.00

Member Price: $275.00

Rating: Member Average


This nine-course program was developed for clinical laboratory professionals with experience using tandem mass spectrometry (LC-MS/MS) and liquid chromatography mass spectrometry (LC-MS) for laboratory analyses. It would benefit technologists who wish to improve their technical skillset, as well as directors or supervisors who would like a course on issues that affect mass spectrometry testing operations. The content of this program is at the intermediate level and covers the resolution of technical issues with HPLC and mass spectrometry hardware and related mass spec assay troubleshooting and assay maintenance, quality control as well as common post-analytical problems. This program is appropriate for learners who have first-hand exposure to the technology through prior use.

The program is composed of nine courses, listed below. Each course can be completed online in 1-3 hours and contains a lecture, reference materials and a quiz. Communicate questions or comments using the program discussion board.


  1. System Suitability Testing and Preventive Maintenance
    • Learning Objectives: Review the goal and criteria of system suitability testing and preventative maintenance
    • Speaker: Y. Victoria Zhang, PhD, University of Rochester Medical Center

  2. Sample Preparation
    • Learning Objectives: Learn the sample preparation steps to maintain sample integrity, approach batch building, extraction precision and documentation.
    • Speaker: Brian Rappold, Collinsvile, IL

  3. Liquid Chromatography for Mass Spectrometry: Operations and Troubleshooting
    • Learning Objectives: Review liquid chromatography fundamentals, system suitability tests, LC maintenance and troubleshooting.
    • Speaker: Brian Rappold, Collinsvile, IL

  4. Mass Spectrometry Troubleshooting
    • Learning Objectives: Identify and use QA tools to develop troubleshooting and maintenance plans for mass spectrometry technology.
    • Speaker: Alan Rockwood, PhD, ARUP Laboratories

  5. Gas Chromatography Mass Spectrometry
    • Learning Objectives: Discuss the components of gas chromatography mass spectrometry, its advantages and limitations, as well as troubleshooting techniques.
    • Speaker: Uttam Garg, PhD, Mercy Children's Hospital

  6. Data Review: Going from Peaks to Numbers
    • Learning Objectives: Learn best practices for integrating peaks and how to implement protocols for manual peak integration. Understand how to calculate and assess relevant quality assurance metrics, and how much data to review.
    • Speaker: Joshua Hayden, PhD, Weill Cornell Medical Center

  7. Quality Control and Quality Assurance for LC-MS/MS; Hydrolysis and Derivatization
    • Learning Objectives: Identify sources and types of QC matrix best suited to LC-MS/MS assays, and list quality indicators for data analysis. Identify the inherent QA/QC challenges of deconjugation and derivatization reactions, and specify appropriate strategies to work around these.
    • Speakers: Julia Drees, PhD, and Sarah Shugarts, PhD, Kaiser Permanente

  8. Troubleshooting Calibration for Clinical Mass Spectrometry
    • Learning Objectives: Understand the range of analytical components that affect calibration of assays, describe the implications of nonlinear calibration curves, and identify steps in calibrator preparation and storage that affect accuracy.
    • Speaker: Stephen Master, MD, PhD, Weill Cornell Medical Center

  9. Dealing with Change
    • Learning Objectives: Describe how clinical LC-MS/MS labs handle lot changes, instrument service and decommission, longitudinal and source material changes, analytical changes in testing methodology, changes to software and personnel.
    • Speaker: Autumn Breaud, PhD, Johns Hopkins University

Launch Year: 2016
Course Number: 12428