The applications of flow cytometry are broad and can be used across a variety of medical areas. One advantage of flow cytometry is a high level of sensitivity compared with other conventional methods of analysis.
This is a basic to intermediate-level online certificate program, which will provide participants with essential knowledge about the use of flow cytometry as a diagnostic tool for the identification of various human diseases. It is intended for laboratory professionals (medical technologists, laboratory managers, laboratory directors), pathologists, core flow cytometry operators, and research scientists who are using flow cytometry in their research. Participants will learn the basics of flow cytometry and how it can be used for diagnosis.
The program is composed of six courses, listed below. Each course can be completed online in approximately 1-2 hours and contains a lecture, slides and transcripts, and a quiz. Some include recommended readings. Enrollees can communicate questions or comments using the program discussion board.
- Basic Theory and Clinical Applications in Flow Cytometry
Susan Orton, PhD, D(AMBLI), University of North Carolina School of Medicine, Chapel Hill, NC
Explains instrumentation used in flow cytometry; discusses the importance of quality assurance in flow cytometry; and lists clinical applications of flow cytometry.
- Introduction to Quality Control and Quality Assurance in Flow Cytometry
Lawrence Eck, NeoGenomics Laboratories, Fort Myers, FL
Discusses regulatory requirements for flow cytometry; explains how sample preparation and instrument function are related to quality control and quality assurance.
- Flow Cytometry Protocols for Immunophenotyping
Roberta Montgomery, MLS, Hennepin County Medical Center, Minneapolis, MN
Lists the methods of immunophenotyping; discusses the requirements for peripheral blood subsets; describes how CD4 counts are used in clinical utility.
- Flow Cytometry for the Analysis of Hematopoietic Neoplasias
Antony Bakke, PhD, D(AMBLI), Clarient, A GE Company
Discusses the application of flow cytometry for hematopoietic neoplasias; explains side scatter and front scatter in flow cytometry plots.
- Flow Cytometry for the Diagnosis of Primary Immunodeficiencies
Roshini Abraham, PhD, D(AMBLI), Mayo Clinic, Rochester, MN.
Describes primary immunodeficiencies; lists the advantages of flow cytometry in primary immunodeficiencies; discusses the differences in primary immunodeficiencies between the adaptive and innate immune systems.
- Flow Cytometry in Transplantation: Antibody Screening and Crossmatching
John Schmitz, PhD, University of North Carolina Hospitals, Chapel Hill, NC
Explains flow cytometric methods for antibody screening and crossmatching in solid organ transplantation.
Launch Year: 2012
Course Number: 12426