On the Clinical Chemistry Facebook page, we received an interesting query from reader Ivo Emo Andrew regarding the effect of storage on Alanine Aminotransferase.

effect of storage on Alanine Aminotransferase - facebook question

As you can see, I attempted to direct Ivo to one of our papers from Ruby et al. Ivo sent me an email with some follow-up questions. I thought that posting these questions to the CouncilChatters might help Ivo find the answers he is looking for.

"Sir, I thank you for the material sent to me. It has widen my knowledge and has ignited lots of question in me.

  1. The work was done couple of decades ago (1988). which is a long period.
  2. Period of research was 72 hours. What happens after that? say a week, 3 weeks, 6 weeks, 6 months....
  3. I agree that there was no significant difference statistically in the results for this time interview. What if time is increased?
  4. Discussions recommend freezing and longer time interval.

Certainly, we know that temperature and time are factors that affect enzyme activity in biochemical reactions.We are suppose to preserve biological fluid and store until when it is needed. The ability to obtain as nearly the same value after storage gives one a reliable and confidence on what we are doing.

Most at time we are concerned with increased in temperature without noticing the effect of the storage precautions."

Does anybody have relevant advice for Ivo?​