Toward Standardization in Protein Quantification Workflows

  • Duration:
  • Date:DEC.8.2016 12:00 AM

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Sample preparation is one of the greatest sources of variability in bioanalytical and clinical research methods, particularly for protein quantification methods which often contain multiple steps; each one contributing to variability.

The issue of variability is of particular concern when test methods are transferred between sites, or even between labs within the same organization. And laboratorians are well aware that the resulting method must meet acceptable accuracy and precision guidelines as well as provide reliable and reproducible results for critical decision-making.

This webinar will focus on the implementation and benefits of generic, kitted methods to streamline and standardize common protein quantification workflows, while reducing variability and delivering accurate results. Several examples will be provided to demonstrate the impact of these methods on controlling and reducing variability.

Target Audience

Lab directors, lab managers, clinical laboratory scientists, and laboratory technicians involved with transferring protein quantification from immunoassay to LC/MS, or those new to protein quantification by LC/MS. Scientists and other professionals who want to better understand the fundamentals of protein quantification by LC/MS and how to implement a reliable standardized approach will also benefit from this webinar.

Learning Objectives

After attending this webinar, you should be able to:

  • Understand and appreciate the importance of standardization in protein quantification
  • Have a working knowledge of a thoroughly researched approach to absolute protein quantification Describe affinity purification options for protein level purification and SPE options for signature peptide purification
  • Evaluate and select the appropriate level of sample preparation, and specific type of sample prep depending on the sensitivity requirements of your assay
  • List the steps of digestion workflows and explain the impact on final quantitative results


photograph of erin chambersErin Chambers, PhD
Director, Technology Advancement Group and Principal Scientist
Waters Corporation
Milford, MA

Registration Information

  • Registration covers one phone line and one computer connection for the webinar.
  • If you are unable to register online, use the PDF registration form provided and fax or mail this form with your payment.
  • Several business days prior to the webinar, AACC will e-mail instructions to the registrant on how to participate, including a phone number to call to listen to the audio portion of the webinar and a URL where you can log in for the visual portion of the webinar.

Technical Requirements

AACC uses WebEx to conduct its webinars. To attend the webinar, you will need:
  • A separate telephone line for the audio or a sound card and speakers
  • A 56Kbps Internet connection or better
  • Windows Users - Internet Explorer 6/7, Firefox 2, Mozilla 1.7+, Netscape 8.1 or higher.
  • Mac Users - Firefox 2, Safari 3.0 (Mac OS 10.4,10.5), Safari 2.0 (Mac OS 10.4) Safari 1.3 (Mac OS 10.3).
  • Linux Users - Firefox 2, Mozilla 1.7 or higher.

NOTE: The WebEx plug-in will not install if your browser has high security settings or you do not have administrative rights on your PC. To test your system, please try joining a test meeting prior to your webinar at: https://www.webex.com/lp/jointest. Once on the site, enter your name and email address and click "join."

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