What, in your opinion, has been the most important contribution you have made to the field of laboratory medicine?
This is a difficult question to answer but I can tell you that these are diverse contributions.
I was the first to discover a new mechanism for the degradation of S-nitrosothiols (S-nitrosocystiene and S-nitrosoglutathione). These are the reservoirs for the storage of nitric oxide in blood. My findings, which were published in the Journal of Biological Chemistry, showed for the first time that nitric oxide, a known vasodilator, is released from its reservoirs in nitrosothiols by superoxide radical. This was the first report to show that superoxide, a free radical, is responsible for the biological release of nitric oxide from nitrosocysteine and nitrosoglutathione. This paper is still quoted by many reports in high impact journals today. The journal of the Proceedings of the National Academy of Science has cited my research in an article edited by Lousi Ignarro, Nobel Laureate in chemistry. The mechanism was confirmed by many subsequent reports including a devoted article by Dr. Peter Wardman of King's College London, published in Journal of Biological Chemistry.
I also coauthored a paper with national and international experts in laboratory medicine "Decoding Laboratory Test Names: A Major Challenge to Appropriate Patient Care". This is an important paper because it highlights and set the stage for the need to improve and standardize test names, a challenge to all health care providers from physician setting level to laboratory bench site.
I also conducted the very early experiments to validate HE4, a test to monitor for recurrence and disease progression in patients with epithelial ovarian cancer. These experiments helped move the test into an automated analyzer and now is available to providers.
I continued to conduct research in cystic fibrosis and search for therapy using fatty acids supplementations. In addition, I'm interested in studying Khat which has cathinone as one of its ingredients associated with many street drugs.