A blood sample from a 39-year-old female patient with newly diagnosed type 2 diabetes was assayed for hemoglobin (Hb) A1c on a Bio-Rad D10 HPLC platform. No peak for A1c was seen on the chromatogram (Fig. 1A), and the result was reported as 0%. A complete blood count was clinically significant for an Hb concentration of 10.9 g/dL (reference interval 12–15 g/dL) and a mean corpuscular volume of 77 fL (reference interval 80–96 fL).
- What was the reason for the absent A1c peak?
- Can A1C be measured in this patient by an alternative method?
- What other factors can affect A1c measurement?
Read the article here for the answers.