2005 Oak Ridge Abstracts

37th Annual Oak Ridge Conference - Pushing the Technology Envelope II, An Exploration of the Future of Clinical Laboratory Testing

Quantitative Diagnostic Technology for Therapeutic Cell Tracking.
Gromen, E., BioPhysics Assay Laboratory, Inc., Worchester, MA.

A reconfigured aca for detecting amplified nucleic acid (DNA/RNA).

Lipscomb, J., JHL Associates, Inc., Mendenhall, PA.

A whole blood point-of-care test for white blood cell count.

Law. W.T., Nikolyukin, Y., Nikolyukin, I. PortaScience Inc., Moorestown, NJ.

A rapid one-step immunochromatographic test for the detection of cobra venom.

Hung, D. Taichung Veterans General Hospital, Taichung City, Taiwan.

A Candidate-Based Approach to Plasma Biomarker Validation Using Quantitative Mass Spectrometry.

Anderson, L. Plasma Proteome Institute, Washington, DC.

Development of an automated enzymatic cycling assay for detection of total homocysteine in human serum or plasma.

Xia, D.Y., Dou, C., Zhang, L.Q., Datta, A., and Yuan, C. Diazyme Laboratories Division, General Atomics, San Diego, CA.

Development of an automated enzymatic coupling assay for detection of lithium ion in human serum.

Dou, C., Aleshin, O., Datta, A., and Yuan, C. Diazyme Laboratories, a Division of General Atomics, San Diego, CA.

Development of an automated enzymatic assay for detection of glycated serum protein in human serum.

Wang, Y.P., Dou, C., Datta, A. and Yuan, C. Diazyme Laboratories Division, General Atomics, San Diego, CA.

Development of an automated enzymatic assay for detection of HbA1c in human whole blood.

Maylin, R., Wang, Y.P., Datta, A., Dou, C., and Yuan, C. Diazyme Laboratories Division, General Atomics, San Diego, CA.

Highly sensitive immunoassays utilizing DNA-conjugated antibodies and liquid-phase binding reaction in a microchip.

Watanabe M.1, Kawabata T.1, Satomura M.1, Kurosawa T.1, Wada H.G.1, Kazakova I.2, Simeonov A.2, Park C.2, Kechagia P.2, and Satomura S.1. 1Wako Pure Chemical Industries Ltd., Osaka, Japan, and 2Caliper Life Sciences, Mt. View, CA.

Plasmonic Technology: Novel Approach to Ultrasensitive Immunoassays.

Evgenia G. Matveeva*, Ignacy Gryczynski*, Joanna Malicka*, Joanna Lukomska*, Slawomir Makowiec*, Robert H. Christenson**, Joseph R. Lakowicz*, and Zygmunt Gryczynski* *Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, MD. ** Rapid Response Laboratories, Department of Pathology, University of Maryland School of Medicine, 22 S. Greene Street, Baltimore, MD 21201; phone: 410-328-8672, fax: 410-328-5880

Analytical Evaluation of HgbA1c, Microalbumin, CRP, and RF on Architect ci8200 Integrated System and Workflow Computer Simulation.

Elizabeth C. Elefano1, Rukshana Jabeen1, A.O. Okorodudu1, J.R. Petersen1, Mayra Vazquez2, David Armbruster2, Keith Chaitoff2, and Amin A. Mohammad1. 1University of Texas Medical Branch, Galveston, TX and 2Abbott Laboratories, Abbott Park, IL.

Evaluation of glycated hemoglobin (HbA1c) measurement with a fully automated POC instrument in comparison with HPLC and automated immunochemistry method.

Haliassos A.1, Makris K.2, Drakopoulos I2, Katritsis D.3, Raptis G.4, Chiotinis N.1. 1Central Laboratories, Athens Euroclinic, Athens, Greece, 2Clinical Biochemistry Laboratory, K.A.T. General Hospital, Kiffisia, Greece, 3Cardiology Department, Athens Euroclinic, Athens, Greece and 4Bayer Diagnostics, Athens, Greece.

Renal mass and mRNA for nephrin.

Orlandi, E1, Butt, A1, Goldsmith, D2, Swaminathan, R1. Departments of 1Chemical Pathology and 2Nephrology, Guy’s and St Thomas’ Hospitals, London, United Kingdom.

Preparation of complex protein and peptide samples for mass spectrometry analysis using magnetic bead technology.

Gillooly, D.J., Breivold, E., Evensen, K., Keiserud, A., Korsnes, L., Bosnes, M., Fonnum, G., Lillehaug, D. Dynal Biotech ASA, Oslo, Norway.

A Multiplexed Immunoassay For Rapid Detection Of Specific Ige In Allergy Diagnosis.

Nystrand M., Elfverson G., Lidholm J., Matsson P., Holmquist S., Pharmacia Diagnostics AB, Uppsala, Sweden.

Effects of fluorinated reagents on immunoassay performance.

Kuerzinger, K., Gloss, A. Roche Diagnostics GmbH, Penzberg, Germany.

Application of multivariate probabilistic (Bayesian) networks to the interpretation of diagnostic assays.

Robin, HS., Eberhardt, JS., Gaertner, R., Armstrong, M. Sharp Memorial Hospital, San Diego, CA.

Personalized anticancer chemotherapy based on measurement of drug response indicators (DRI) expressed by cancer cells.

Ts’o, P.O.P.1, Lesko, S.A.1, Lum, Z.P.1 , Deamond, S.F.1, Shan, E.1, Zhou, D.B.1, Daniel, J.R.1. 1 CCC Diagnostics, LLC, Baltimore, MD.

Determination of glycation on diabetic erythrocytes by electromagnetophoresis for prediction of aggregation of erythrocytes in the vessels of diabetic patients.

Nozaki, O. Munesue, M., Kawamoto, H. Dept of Clin Lab Med, Kinki Univ School of Medicine. Osaka-Sayama, Japan.

Multiplex assay systems based on superparamagnetic fluorescent Dynabeads.

Egeberg, M., Modahl, G., Weng, E., Molteberg, A., Stene, T., Manger, I., Korsnes, L., Fonnum, G., Lillehaug, D. Dynal Biotech ASA, Oslo, Norway.

New magnetic bead surfaces optimized for high sensitivity immunoassays.

Pringle S, Songe P, Borgen T, Aksnes E, Stene T, Tran D, Hofsløkken N, Fonnum G, Trømborg H., Dynal Biotech ASA, Oslo, Norway.

An integrated microfluidic CD device with potential utility both in centralized- and POC laboratory settings.

Inganäs M., Derand H., Eckersten A., Honerud A.-K., Thorsén G., Söderman T., Andersson P. Gyros AB, Uppsala, Sweden.

Novel homogeneous assay technology based on up-converting nanophosphors.

Soukka, T. Kuningas, K. Rantanen, T. Ukonaho, T. Lovgren, T. University of Turku, Turku, Finland.

Enhanced Raman-encoded nanobeads for ultra-sensitive labeling and multiplexed detection of nucleic acids.

Corish, P., Holland, E.R., Lipscomb, S.J., Prince, P.D. & Wakefield G. Oxonica Ltd., Oxford, UK.

Detection of bladder cancer using quantitative reverse transcriptase polymerase chain reaction of urinary

Survivin. Li, Z. Geschwindt, R.G. Kenney, D.M. Sardesai, N.Y. Fujirebio Diagnostics, Inc., Malvern, PA.

Malarial diagnosis using spectral acquisition process detection (SAPD) technology.

Garcia-Rubio,L.G.1, Alupoaei,C.1, Huffman,D.E.1, Klungness,G.1, Leparc,G.2, 1Univ. of South Florida,St. Petersburg, Fl. 2Florida Blood Services, St. Petersburg, FL.

High Resolution Hydration analysis as a Tool for the early Diagnosis of misfolding Disorders.

Funck. T1, Bierbaum H.1, Dickopf S.1, Fagan A.2, Schmitt J.3, and Holtzman D.2. 1TF Instruments GmbH, 69120 Heidelberg, Germany; 2Washington University School of Medicine St. Louis, MO, USA; 3Synthon GmbH, Heidelberg, Germany.

Indirect immuno-PCR (i2PCR): An update on progress in development.

Banin S., Wilson S., Stanley C. ISEAO Technologies, London, UK.

The pharmacokinetics of an albumin binding Fab (AB.Fab) can be modulated as a function of affinity for albumin.

Allen Nguyen1, Arthur Reyes2, Min Zhang3, Paul McDonald4, Wai Lee Wong1, Lisa A. Damico2 and Mark S. Dennis3 1Assay & Automation Technology, 2Early Development PKPD, 3Antibody Engineering Department and 4Process Sciences, Genentec, Inc. San Francisco, CA.

A recombinant cell-based bioluminescent assay for androgen bioactivity determination in human serum.

Michelini E.1, Leskinen P.2, Virta M.2,3, Karp M.2,4, Roda A.1. 1Department of Pharmaceutical Sciences, University of Bologna, Bologna, Italy, 2Department of Biotechnology, University of Turku, Turku, Finland, 3Department of Applied Chemistry and Microbiology, University of Helsinki, Helsinki, Finland, 4Tampere University of Technology, Tampere, Finland.

A rapid chemiluminescent immunoassay format for simultaneous detection of Escherichia coli O157:H7, Yersinia enterocolitica, Salmonella typhimurium, and Listeria monocytogenes pathogen bacteria.

Magliulo M.1, Mirasoli M.1, Simoni P.2, Lelli R.3, Luciani M.3, Roda A.1. 1Department of Pharmaceutical Sciences and 2 Department of Internal Medicine and Gastroenterology, University of Bologna, Bologna, Italy 3 Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise, Teramo, Italy.

A flow-assisted, solid-phase immunoassay with chemiluminescence detection for multianalyte platforms.

Roda B.1, Melucci D. 1, Mirasoli M.2, Reschiglian P.1, Roda A.2.1Department of Chemistry "G. Ciamician", University of Bologna, Bologna, Italy, 2Department of Pharmaceutical Sciences, University of Bologna, Bologna, Italy.

Mulitplexed Assay for the Detection of Biowarfare Agents in Water-Borne Samples.

R. Hydutsky, R. Street, M. Odom, D. Qin, J. Pan, R. Daniel, J. Jensen*, and R. Yin. ANP Technologies, Inc., Newark, DE. * U.S. Army Edgewood Chemical Biological Center Aberdeen Proving Ground, MD.

Multiplexed Cytokine Assay for examining the TH1 / TH2 Paradigm.

H. Knapp, S. Gibbs, J. Pan, and R. Yin., ANP Technologies, Inc., Newark, DE.

Nucleic acid detection immunoassay (NADIA).

Jablonski E.G., Adams T.A. Leucadia Technologies, Inc., Carlsbad, CA.

Electroactive Lab on Card (eLab) : a plastic disposable diagnostic card with DNA electric detection, pyrotechnic valves and a microfluidic network for biological testing.

Foucault F.1, Broyer P.1, Ginot F.1, Menou L.1 et al., Roller D.2, Gaud C.2, Campagnolo R.3, Marchand G.3, Pouteau P.3, Calvas B.4 et al. 1bioMérieux, Lyon, France, 2SME, Paris, France, 3CEA, Grenoble, France, 4SPS, Marseille, France.

High evaluable rate and specificity of the prototype aptima pca3 molecular urine test.

Leonard Marks1, Kumar Kastury2, Craig Clark2, Amy Blase2, Harry Rittenhouse2, Sharon Bodrug2, Eric Rasmussen2, Jeannette Mathis2, Jimmykim Pham2, Zephyr Fors2, Steven Brentano2 1 Urological Sciences Research Foundation, Culver City, CA and 2Gen-Probe Incorporated, San Diego, CA.

Homocysteine and folate in human serum: development of a tandem mass spectrometry reference method and standard reference material for emerging cardiovascular disease markers.

Nelson, B.C.1, Satterfield, M.B.1, Sniegoski, L.T.1, Hornikova, A.2, Welch, M.J.1 1NIST, Analytical Chemistry Division, Gaithersburg, Maryland and 2NIST, Statistical Engineering Division, Gaithersburg, MD.

Aptima pca3 molecular urine test: Development of a method to aid in the diagnosis of prostate cancer.

Craig Clark1, Harry Rittenhouse1, Sharon Bodrug1, Steven Brentano1, Eric Rasmussen1, Jeannette Mathis1, Jimmykim Pham1, Marie Desaulniers2, Guillaume Beaudry2, Camille Chypre2, Lyson Piche2, Yves Fradet2 1Gen-Probe Incorporated, San Diego, CA and 2 Diagnocure, Sainte-Foy(Quebec), Canada.

Immunogenicity testing for antibodies directed against therapeutic human monoclonal antibodies using electrochemiluminescent detection.

Moxness, M.S., Tatarewicz, S., Weeraratne, D., Murakami, N., Mytych, D., Jawa, V., Koren,E., Swanson, S. Clinical Immunology, Amgen, Inc., Thousand Oaks, CA.

Identification of Dengue Virus serotype 1-4 using lateral flow testing device after Ready-To-Use (RTU) PCR reaction.

Jung,J., Kim,Y.S., Baek,S.J., Choi, B.K., Kim,S. and Choi,Y.H., Access Bio Inc., NJ.

A supersensitive lateral-flow platform for the detection of P. falciparum malaria using the luminescent europium nanoparticle.

Choi, Y.H. 1, Choi, B.K.1, Kim, H.1, Baek, S.J. 1, Jung, J. 1, Makler, M.T. 2, 1Access Bio, Inc., Monmouth Jct., NJ and 2Flow Inc., Portland, OR.

Microcytometer lab card for rapid antibody labeling, counting, and sorting of rare blood cells.

Gerdes J. , Lancaster C. , Kokoris M., Nabavi M. , Clemmens J. , Maloney P. , Capadanno J. , Battrell C.F., Micronics Inc., Redmond, Washington.

A Multimarker Index methodology improves the diagnosis and prognosis of complicated diseases.

Anderberg J., Buechler K., McPherson P., Dahlen J., Whittaker M. Biosite, San Diego, CA.

A sensitive, nested real-time RT-PCR duplex assay for quantitation of BCR-ABL and ABL.

Wang J.Q., Paul K.L., Wong W.W., Helton B., Van Atta R., Ching J.. Cepheid, Sunnyvale, CA.

Pre-clinical data show Theranostech, Tagger HIV-1 test detects and indexes HIV infectivity status in patients.

Adrian Rubio1, Bert Davis Ph.D.2, Kristine Garver1 and Jess Stengel1. 1 Theranostech, Incorporated, 2 Independent Statistical Consultant. Albuquerque, NM.

Haploseparation and amplification of genomic DNA by haplotype-specific extraction (HSE) and whole-genome amplification (WGA).

Dapprich, J. 1, Turino, C. 2, Scavello, G. 2, McCarro, V. 2, Murphy, C. 2, Murphy, N. 2 . 1 Generation Biotech, Lawrenceville, NJ 2 GenoVision, West Chester, PA.

Single nucleotide polymorphism analysis without target amplification using planar waveguide technology.

Burmeister, J.1, Dorn, I.1, Warner, B. D.2, Bazilyanska, V. 1, Booth Johnson, J. 2, Shermoen, D. 2, Wong, C. 2, Raczek, U. 1, Leisner, W. 1. 1Bayer Technology Services GmbH, Competence Center Biophysics, Leverkusen, Germany and 2Bayer Diagnostics, Berkeley, CA.

Development and Optimization of Surfaces and Antibody Ligation Methods for Immunoassays using High Throughput Discovery Platform.

Monteiro R.L., Muir B.W., Collett S.P., Maeji N.J., Gorse A.D. Bio-Layer, 4/26 Brandl Street, Brisbane, QLD 4113, Australia.

Multiplexed Quartz Crystal Microbalance for the Determination of Coagulation Kinetics & Endotoxin: A Precursor for Sepsis Testing.

G. Klivenyi, I. Horvath, A. Istokovics, K. Revesz, B. Jancsik. OPULUS Ltd., Philadelphia, PA.

Electrochemiluminescent multiplexed measurement of biomarkers: current applications in drug discovery and future applications in the clinic.

Glezer E.N., Debad J.D., Abdu A., Wohlstadter J.N. Meso Scale Diagnostics, Gaithersburg, MD.

Large Multi-Analyte Profile (MAP) Analyses Used to Identify Biomarkers Attributable to Extreme Physical Exercise.

Mapes J.P., Kemp, J.L., R.L., Sandefur, M., Casey, C., Denton, D., Gamboa, M., Frazier, D., Bartaszewicz, L. Rules-Based Medicine, Inc., Austin, TX.

A real-time exhaled breath assay for detection of Helicobacter pylori using a colorimetric sensor array based technology.

Hulkower, K.I.1, Sen, A.1, Dryer, J.S.1, Nedrud, J.G.2. 1ChemSensing, Inc., Northbrook, IL and 2Case Western Reserve University, Cleveland, OH.

Novel Isothermal, linear nucleic acid amplification systems for highly multiplex applications.

Kurn N, NuGEN Technologies Inc., San Carlos, CA.

Lyotropic liquid crystals as a novel solid phase for immunoassays.

Mitchell, JE1, Saul, RG2, Anderson, DA1, 1Lyotropic Diagnostics, Richmond, VA and 2Axo Diagnostics, Gaithersburg, MD.

Spatial and temporal imaging of surface binding events using phase differences of surface plasmon resonance.

Day, R.F.1, French, M.T.1, Greenwood, J.A.1, Hazell, M.S.1, Jones, R.1, McKnight, D.1, Athey, D.2, Lakey, J.2, Shah, D.2 1Cambridge Consultants Ltd, Cambridge UK and 2Orla Protein Technologies, Newcastle-upon-Tyne UK.

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