Follow Clinical Chemistry On:
ON THE COVER: Droplet digital PCR. In this new generation of PCR technology, droplet microfluidic systems are used to create and analyze nanoliter to picoliter droplets, with the result that these processes enable simple digital PCR work flows that yield highly sensitive mutation detection within complex DNA mixtures. This issue of Clinical Chemistry contains 2 original research articles that illustrate the application of digital droplet PCR for measuring cell-free nucleic acids. In the first article researchers use this technology to detect KRAS mutations in circulating DNA from the plasma of patients with colorectal cancer. In the second article researchers use digital droplet PCR for the rapid quantification of donor DNA in the circulation of transplant recipients as a potential biomarker of graft injury. An accompanying editorial highlights both of these articles. (See pages 1691, 1722, and 1732.) ©Bio-Rad Laboratories. Reproduced with permission.