American Association for Clinical Chemistry
Better health through laboratory medicine
August 2008 Mentor of the Month: Pratap Singh
Pratap Singh, PhD

Dr. Pratap Singh, is a Dade Behring Research Fellow and Principal Scientist in the assay development group of a Siemens Healthcare Diagnostics facility located in Glasgow, Delaware.

He received his B. Sc. (Hons.), M. Sc. and PhD from University of Delhi in organic chemistry. Between 1971 and 1974, Dr. Singh was involved in the drug discovery program at Central drug Research Institute in Lucknow, India. He was a post doctoral fellow at the University of Chicago, Fordham University and University of Michigan at Ann Arbor and a research fellow at Henry Ford Hospital.

Dr. Singh has been on the staff of Siemens Healthcare Diagnostics and its predecessor firms since 1984. His research interests include bio-conjugation, protein modification and characterization and organic synthesis. In the early 1990s, while at Dade in Miami, Florida, he conceived utilization of dendrimers, a well defined nanoscopic synthetic polymer, in controlling orientation of immobilized immuno-reagents for the assays requiring very low detection limits and excellent precision. He and his team experimentally demonstrated the feasibility for this concept using polyamidoamine  (PAMAM) dendrimers. By employing this concept it was possible to reproducibly achieve highly specific and sensitive assays for important analytes like creatine kinase MB subunit, thyroxine and human thyroid stimulating hormone. This work eventually led to the development of Stratus® CS, an automated clinical analyzer, with capability to analyze six key analytes including a number of cardiac markers such as cardiac troponin I and N-terminal pro-brain natriuretic peptide. These and other sensitive assays on Stratus® CS have found great utility in emergency room and critical care settings in which quick decisions for cardiac and other patients are needed.

He is a co-inventor on eight patents relating to clinical diagnostics, a contributing author of a chapter in a book on dendrimers and has published over twenty research articles in the fields of clinical and organic chemistry. He has participated as an invited speaker on two dendrimer symposia and was interviewed by Chemical and Engineering News for a feature article about dendrimers. He is a member of the Protein Society and American Chemical Society.