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. Well, over the invening years, there still were some pareps on this topic, mainly dealing with antisense oligonucleotides, siRNAs, ribozymes, CpG oligonucleotides, as a ligand for the assay. But I think, the present publication once again shows the power and usability of hybridisation assays in general, even if it's applied to microRNA therapeutics (both mimics and antagomirs), and even the Real-Time RT-qPCR ligand binding assay should exhibit an even better sensitivity. it's also one of the first to provide proof-of-concept to in vivo pharmacokinetic studies, although the ligation-based method assays only parent compound, but not 3b4-truncated drugs, as the T4 DNA ligase can not ligate this metabolites. Please have in mind these metabolites can still be bioactive.Have a nice weekend, your microRNA-bioanalytics.de team
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