Basic Concepts and Clinical Applications of Flow Cytometry

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Earn 12 ACCENT® continuing education credits and a certificate of completion for successfully completing the program before the access end date.

The program is composed of six courses, listed below. Each course can be completed online in approximately 2 hours and contains a lecture, slides, transcript, and a quiz. Some include additional reading materials. Communicate questions or comments using the program discussion board.

  • Basic Theory and Clinical Applications in Flow Cytometry
  • Introduction to Quality Control and Quality Assurance in Flow Cytometry
  • Flow Cytometry Protocols for Immunophenotyping
  • Flow Cytometry for the Analysis of Hematopoietic Neoplasias
  • Flow Cytometry for the Diagnosis of Primary Immunodeficiencies
  • Flow Cytometry in Transplantation: Antibody Screening and Crossmatching

Certificates are awarded online upon successful completion of the program. Graduates are recognized on a register located on the certificate program web page.


The applications of flow cytometry are broad and it can be used across a variety of medical areas. One advantage of flow cytometry is a high level of sensitivity compared with other conventional methods of analysis.

This a basic to intermediate-level online certificate program, which will provide participants with essential knowledge about the use of flow cytometry as a diagnostic tool for the identification of various human diseases. It is intended for laboratory professionals (medical technologists, laboratory managers, laboratory directors), pathologists, core flow cytometry operators, and research scientists who are using flow cytometry in their research. Participants will learn the basics of flow cytometry and how it can be used for diagnosis.

After completing this program, participants will be able to:

  • Describe the basic principles of flow cytometry
  • Discuss the regulatory requirements for quality control in flow cytometric assays
  • Understand the technical and software requirements for designing of flow cytometric protocols for diagnostic purposes
  • Explain the use of flow cytometry in immune-mediated disorders

These expert faculty developed and contributed content to the program:

  • Gabriella Lakos, MD, PhD, Faculty Chair
    Inova Diagnostics Inc, San Diego, CA
  • Vijaya Nagabhushanam, PhD, Faculty Chair
    National Jewish Health, Denver, CO
  • Evan Ntrivalas, MD, PhD, HCLD(ABB), D(ABMLI), Faculty Chair
    Foundation for Blood Research, Scarborough, ME
  • Roshini Abraham, PhD, D(ABMLI), FAAAAI
    Mayo Clinic, Rochester, MN
  • Antony Bakke, PhD, D(ABMLI)
    Clarient, A GE Healthcare Company
  • Lawrence Eck
    NeoGenomics Laboratories, Fort Myers, FL
  • Roberta Montgomery, MLS, MLS(ASCP)SI
    Hennepin County Medical Center, Minneapolis, MN
  • Susan Orton, PhD, D(ABMLI)
    University of North Carolina School of Medicine, Chapel Hill, NC
  • John Schmitz, PhD
    University of North Carolina Hospitals, Chapel Hill, NC

Developed in cooperation with the AACC Clinical and Diagnostic Immunology Division and AMLI.


    Open Enrollment Until December 31, 2014
    Program Access from January 1, 2014 until July 15, 2015

    Fee: $275 AACC Member/$550 Non-member
    Course Number: 8735
    12 ACCENT® continuing education credits

    • If you purchase the program on our website, processing is immediate and the login information will be on your AACC Web Orders confirmation email.
    • If you purchase the program over the telephone, order processing will take 3-4 business days. Access information will be emailed to you upon processing completion/enrollment.
    • If you purchase the program via US Post or fax, please allow 2 weeks for order processing.
    • Haven’t received your access information? Please contact AACC Customer Service.  


    ACCENT® credit is offered. AACC is an approved provider of continuing education for clinical laboratory scientists in the states of California, Florida, Louisiana, Montana, Nevada, North Dakota, Rhode Island, and West Virginia.

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